Quantifying stretching and rearrangement in epithelial sheet migration
Name
Lee-2013-Quantifying stretchi.pdf
Size
1.74 MB
Format
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Checksum (MD5)
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Author(s)
Lee, Rachel M.
Kelley, Douglas H.
Nordstrom, Kerstin N.
Ouellette, Nicholas T.
Losert, Wolfgang
Date Issued
February 2013
Journal
New Journal of Physics
Publisher
IOP Publishing
Citation
Lee, Rachel M et al. “Quantifying Stretching and Rearrangement in Epithelial Sheet Migration.” New Journal of Physics 15.2 (2013): 025036. ©2013 IOP Publishing
Version
Final published version
Abstract
Although understanding the collective migration of cells, such as that seen in epithelial sheets, is essential for understanding diseases such as metastatic cancer, this motion is not yet as well characterized as individual cell migration. Here we adapt quantitative metrics used to characterize the flow and deformation of soft matter to contrast different types of motion within a migrating sheet of cells. Using a finite-time Lyapunov exponent (FTLE) analysis, we find that—in spite of large fluctuations—the flow field of an epithelial cell sheet is not chaotic. Stretching of a sheet of cells (i.e. positive FTLE) is localized at the leading edge of migration and increases when the cells are more highly stimulated. By decomposing the motion of the cells into affine and non-affine components using the metric [D[superscript 2] over min], we quantify local plastic rearrangements and describe the motion of a group of cells in a novel way. We find an increase in plastic rearrangements with increasing cell densities, whereas inanimate systems tend to exhibit less non-affine rearrangements with increasing density.
MIT Department
Massachusetts Institute of Technology. Materials Processing Center
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
Creative Commons Attribution 3.0
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DOI of Published Version
https://doi.org/10.1088/1367-2630/15/2/025036
