Mechanical Properties of Tissue-Engineered Vascular Constructs Produced Arterial or Venous Cells
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Gauvin-2011-Mechanical Propertie.pdf
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Author(s)
Gauvin, Robert
Guillemette, Maxime D.
Galbraith, Todd
Bourget, Jean-Michel
Larouche, Danielle
Marcoux, Hugo
Aube, David
Hayward, Cindy
Auger, Francois A.
Germain, Lucie
Date Issued
July 2011
Journal
Tissue Engineering. Part A
Publisher
Mary Ann Liebert, Inc.
Citation
Gauvin, Robert et al. “Mechanical Properties of Tissue-Engineered Vascular Constructs Produced Using Arterial or Venous Cells.” Tissue Engineering Part A 17 (2011): 2049-2059. © Mary Ann Liebert, Inc.
Version
Final published version
Abstract
There is a clinical need for better blood vessel substitutes, as current surgical procedures are limited by the availability of suitable autologous vessels and suboptimal behavior of synthetic grafts in small caliber arterial graft (<5 mm) applications. The aim of the present study was to compare the mechanical properties of arterial and venous tissue-engineered vascular constructs produced by the self-assembly approach using cells extracted from either the artery or vein harvested from the same human umbilical cord. The production of a vascular construct comprised of a media and an adventitia (TEVMA) was achieved by rolling a continuous tissue sheet containing both smooth muscle cells and adventitial fibroblasts grown contiguously in the same tissue culture plate. Histology and immunofluorescence staining were used to evaluate the structure and composition of the extracellular matrix of the vascular constructs. The mechanical strength was assessed by uniaxial tensile testing, whereas viscoelastic behavior was evaluated by stepwise stress-relaxation and by cyclic loading hysteresis analysis. Tensile testing showed that the use of arterial cells resulted in stronger and stiffer constructs when compared with those produced using venous cells. Moreover, cyclic loading demonstrated that constructs produced using arterial cells were able to bear higher loads for the same amount of strain when compared with venous constructs. These results indicate that cells isolated from umbilical cord can be used to produce vascular constructs. Arterial constructs possessed superior mechanical properties when compared with venous constructs produced using cells isolated from the same human donor. This study highlights the fact that smooth muscle cells and fibroblasts originating from different cell sources can potentially lead to distinct tissue properties when used in tissue engineering applications.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1089/ten.TEA.2010.0613
