Lensless imaging for point-of-care testing
Name
SangJun-2009-Lensless imaging for point-of-care testing.pdf
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738.66 KB
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Author(s)
Moon, SangJun
Keles, Hasan Onur
Kim, Yun-Gon
Kuritzkes, Daniel R.
Demirci, Utkan
Date Issued
November 2009
Journal
Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2009. EMBC 2009
Publisher
Institute of Electrical and Electronics Engineers
Citation
SangJun Moon et al. “Lensless imaging for point-of-care testing.” Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE. 2009. 6376-6379. © 2009 IEEE
Version
Final published version
Abstract
We show a platform that merges a microfluidic chip with lensless imaging for CD4[superscript +] T-lymphocyte counting at resource-limited settings. To capture CD4[superscript +] T lymphocytes, anti-CD4[superscript +] antibody was immobilized on a microfluidic chip. The captured cells were detected by a charge coupled device (CCD) sensor using lensless shadow imaging techniques. Gray scale shadow images of captured cells on the chip (24 mm times 4 mm times 50 mum) were enumerated in three seconds using an automatic cell counting software. The device achieved 70.2 plusmn 6.5% capture efficiency, 88.8 plusmn 5.4% capture specificity for CD4[superscript +] T-lymphocytes, 96 plusmn 1.6% CCD efficiency, and 83.5 plusmn 2.4% overall platform performance (n = 3D 9 devices). This integrated platform has potential for point-of-care testing (POCT) to rapidly capture, image and count specific cell types from unprocessed whole blood.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1109/IEMBS.2009.5333765
