Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
Name
Kamm_Microfluidic assay.pdf
Size
2.01 MB
Format
Adobe PDF
Checksum (MD5)
997dc26680e0e4c02fb283fdb41fd8b3
Author(s)
Shin, Yoojin
Han, Sewoon
Jeon, Jessie S.
Yamamoto, Kyoko
Zervantonakis, Ioannis K
Sudo, Ryo
Kamm, Roger Dale
Chung, Seok
Zervantonakis, Ioannis K.
Date Issued
June 2012
Journal
Nature Protocols
Publisher
Nature Publishing Group
Citation
Shin, Yoojin, Sewoon Han, Jessie S Jeon, Kyoko Yamamoto, Ioannis K Zervantonakis, Ryo Sudo, Roger D Kamm, and Seok Chung. “Microfluidic Assay for Simultaneous Culture of Multiple Cell Types on Surfaces or Within Hydrogels.” Nat Protoc 7, no. 7 (June 7, 2012): 1247–1259.
Version
Author's final manuscript
Abstract
This protocol describes a simple but robust microfluidic assay combining three-dimensional (3D) and two-dimensional (2D) cell culture. The microfluidic platform comprises hydrogel-incorporating chambers between surface-accessible microchannels. By using this platform, well-defined biochemical and biophysical stimuli can be applied to multiple cell types interacting over distances of <1 mm, thereby replicating many aspects of the in vivo microenvironment. Capabilities exist for time-dependent manipulation of flow and concentration gradients as well as high-resolution real-time imaging for observing spatial-temporal single-cell behavior, cell-cell communication, cell-matrix interactions and cell population dynamics. These heterotypic cell type assays can be used to study cell survival, proliferation, migration, morphogenesis and differentiation under controlled conditions. Applications include the study of previously unexplored cellular interactions, and they have already provided new insights into how biochemical and biophysical factors regulate interactions between populations of different cell types. It takes 3 d to fabricate the system and experiments can run for up to several weeks.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nprot.2012.051
