Engineering Escherichia coli coculture systems for the production of biochemical products
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Zhang-2015-Engineering Escheric.pdf
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Author(s)
Zhang, Haoran
Li, Zhengjun
Stephanopoulos, Gregory
Pereira, Brian J.
Date Issued
July 2015
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Zhang, Haoran, Brian Pereira, Zhengjun Li, and Gregory Stephanopoulos. “Engineering Escherichia Coli Coculture Systems for the Production of Biochemical Products.” Proc Natl Acad Sci USA 112, no. 27 (June 25, 2015): 8266–8271.
Version
Final published version
Abstract
Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that successfully overcomes fundamental microbial production limitations, such as high-level intermediate secretion and low-efficiency sugar mixture utilization. For the production of the important chemical cis,cis-muconic acid, we show that the coculture approach achieves a production yield of 0.35 g/g from a glucose/xylose mixture, which is significantly higher than reported in previous reports. By efficiently producing another compound, 4-hydroxybenzoic acid, we also demonstrate that the approach is generally applicable for biosynthesis of other important industrial products.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1506781112
