Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion
Name
s41467-023-40858-x.pdf
Description
Published version
Size
4.9 MB
Format
Adobe PDF
Checksum (MD5)
ae65bf548cb22d85321ddbbcfa80584a
Author(s)
Jiang, Chao
Luo, Hong-Yu
Xu, Xinpeng
Dou, Shuo-Xing
Li, Wei
Guan, Dongshi
Ye, Fangfu
Chen, Xiaosong
Guo, Ming
Wang, Peng-Ye
Date Issued
August 24, 2023
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Jiang, Chao, Luo, Hong-Yu, Xu, Xinpeng, Dou, Shuo-Xing, Li, Wei et al. 2023. "Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion." Nature Communications, 14 (1).
Version
Final published version
Abstract
AbstractCell migration plays important roles in many biological processes, but how migrating cells orchestrate intracellular molecules and subcellular structures to regulate their speed and direction is still not clear. Here, by characterizing the intracellular diffusion and the three-dimensional lamellipodium structures of fish keratocyte cells, we observe a strong positive correlation between the intracellular diffusion and cell migration speed and, more importantly, discover a switching of cell migration modes with reversible intracellular diffusion variation and lamellipodium structure deformation. Distinct from the normal fast mode, cells migrating in the newly-found slow mode have a deformed lamellipodium with swollen-up front and thinned-down rear, reduced intracellular diffusion and compartmentalized macromolecule distribution in the lamellipodium. Furthermore, in turning cells, both lamellipodium structure and intracellular diffusion dynamics are also changed, with left-right symmetry breaking. We propose a mechanism involving the front-localized actin polymerization and increased molecular crowding in the lamellipodium to explain how cells spatiotemporally coordinate the intracellular diffusion dynamics and the lamellipodium structure in regulating their migrations.
Subjects
General Physics and Astronomy
General Biochemistry, Genetics and Molecular Biology
General Chemistry
Multidisciplinary
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1038/s41467-023-40858-x
