Scanless volumetric imaging by selective access multifocal multiphoton microscopy
Name
optica-6-1-76.pdf
Description
Published version
Size
2.28 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Xue, Yi
Berry, Kalen Paul
Boivin, Josiah R.
Rowlands, Christopher
Takiguchi, Yu
Nedivi, Elly
So, Peter T. C.
Date Issued
January 2019
Journal
Optica
Publisher
The Optical Society
Citation
Xue, Yi et al. "Scanless volumetric imaging by selective access multifocal multiphoton microscopy." Optica 6, 1 (January 2019): 76-83 © 2019 Optical Society of America.
Version
Final published version
Abstract
Simultaneous, high-resolution imaging across a large number of synaptic and dendritic sites is critical for understanding how neurons receive and integrate signals. Yet, functional imaging that targets a large number of submicrometer-sized synaptic and dendritic locations poses significant technical challenges. We demonstrate a new parallelized approach to address such questions, increasing the signal-to-noise ratio by an order of magnitude compared to previous approaches. This selective access multifocal multiphoton microscopy uses a spatial light modulator to generate multifocal excitation in three dimensions (3D) and a Gaussian–Laguerre phase plate to simultaneously detect fluorescence from these spots throughout the volume. We test the performance of this system by simultaneously recording Ca 2 dynamics from cultured neurons at 98–118 locations distributed throughout a 3D volume. This is the first demonstration of 3D imaging in a “single shot” and permits synchronized monitoring of signal propagation across multiple different dendrites.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Department of Biology
Picower Institute for Learning and Memory
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1364/optica.6.000076
