In vitro blood cell viability profiling of polymers used in molecular assembly
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Author(s)
Jeong, Hyejoong
Hwang, Jangsun
Lee, Hwankyu
Choi, Jonghoon
Hong, Jinkee
Hammond, Paula T
Date Issued
August 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Jeong, Hyejoong et al. “In Vitro Blood Cell Viability Profiling of Polymers Used in Molecular Assembly.” Scientific Reports 7, 1 (August 2017): 9481 © 2017 The Author(s)
Version
Final published version
Abstract
Biocompatible polymers have been extensively applied to molecular assembly techniques on a micro- A nd nanoscale to miniaturize functional devices for biomedical uses. However, cytotoxic assessments of developed devices are prone to partially focus on non-specific cells or cells associated with the specific applications. Thereby, since toxicity is dependent on the type of cells and protocols, we do not fully understand the relative toxicities of polymers. Additionally, we need to ensure the blood cell biocompatibility of developed devices prior to that of targeted cells because most of the devices contact the blood before reaching the targeted regions. Motivated by this issue, we focused on screening cytotoxicity of polymers widely used for the layer-by-layer assembly technique using human blood cells. Cytotoxicity at the early stage was investigated on twenty types of polymers (positively charged, negatively charged, or neutral) and ten combination forms via hemolysis, cell viability, and AnnexinV-FITC/PI staining assays. We determined their effects on the cell membrane depending on their surface chemistry by molecular dynamics simulations. Furthermore, the toxicity of LbL-assembled nanofilms was assessed by measuring cell viability. Based on this report, researchers can produce nanofilms that are better suited for drug delivery and biomedical applications by reducing the possible cytotoxicity.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/S41598-017-10169-5
