CRISPR-based diagnostics
Author(s)
Kaminski, Michael M.
Abudayyeh, Omar O.
Gootenberg, Jonathan S
Zhang, Feng
Collins, James J.
Date Issued
July 2021
Journal
Nature Biomedical Engineering
Publisher
Springer Science and Business Media LLC
Citation
Kaminski, Michael M. et al. "CRISPR-based diagnostics." Nature Biomedical Engineering 5, 7 (July 2021): 643–656. © 2021 Springer Nature Limited
Version
Author's final manuscript
Abstract
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel. Recent developments in diagnostic technologies, in particular those leveraging clustered regularly interspaced short palindromic repeats (CRISPR), aim to enable accurate testing at home, at the point of care and in the field. In this Review, we provide a rundown of the rapidly expanding toolbox for CRISPR-based diagnostics, in particular the various assays, preamplification strategies and readouts, and highlight their main applications in the sensing of a wide range of molecular targets relevant to human health.
MIT Department
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41551-021-00760-7
