Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets
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Pathogenesis and Transmission.pdf
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Author(s)
Pearce, Melissa B.
Jayaraman, Akila
Pappas, Claudia
Belser, Jessica A.
Zeng, Hui
Gustin, Kortney M.
Maines, Taronna R.
Sun, Xiangjie
Raman, Rahul
Cox, Nancy J.
Date Issued
February 2012
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Pearce, M. B., A. Jayaraman, C. Pappas, J. A. Belser, H. Zeng, K. M. Gustin, T. R. Maines, et al. “Pathogenesis and Transmission of Swine Origin A(H3N2)v Influenza Viruses in Ferrets.” Proceedings of the National Academy of Sciences 109, no. 10 (February 21, 2012): 3944–3949.
Version
Final published version
Abstract
Recent isolation of a novel swine-origin influenza A H3N2 variant virus [A(H3N2)v] from humans in the United States has raised concern over the pandemic potential of these viruses. Here, we analyzed the virulence, transmissibility, and receptor-binding preference of four A(H3N2)v influenza viruses isolated from humans in 2009, 2010, and 2011. High titers of infectious virus were detected in nasal turbinates and nasal wash samples of A(H3N2)v-inoculated ferrets. All four A(H3N2)v viruses possessed the capacity to spread efficiently between cohoused ferrets, and the 2010 and 2011 A(H3N2)v isolates transmitted efficiently to naïve ferrets by respiratory droplets. A dose-dependent glycan array analysis of A(H3N2)v showed a predominant binding to α2-6–sialylated glycans, similar to human-adapted influenza A viruses. We further tested the viral replication efficiency of A(H3N2)v viruses in a relevant cell line, Calu-3, derived from human bronchial epithelium. The A(H3N2)v viruses replicated in Calu-3 cells to significantly higher titers compared with five common seasonal H3N2 influenza viruses. These findings suggest that A(H3N2)v viruses have the capacity for efficient replication and transmission in mammals and underscore the need for continued public health surveillance.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. School of Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1073/pnas.1119945109
