Raman spectroscopic sensing of carbonate intercalation in breast microcalcifications at stereotactic biopsy
Name
Sathyavathi-2015-Raman spectroscopic.pdf
Size
1.27 MB
Format
Adobe PDF
Checksum (MD5)
7bb3bb9e17034ec3767bb344a3caa2c7
Author(s)
Sathyavathi, R.
Saha, Anushree
Soares, Jaqueline S.
Spegazzini, Nicolas
McGee, Sasha
Fitzmaurice, Maryann
Barman, Ishan
Dasari, Ramachandra Rao
Date Issued
April 2015
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Sathyavathi, R., Anushree Saha, Jaqueline S. Soares, Nicolas Spegazzini, Sasha McGee, Ramachandra Rao Dasari, Maryann Fitzmaurice, and Ishan Barman. “Raman Spectroscopic Sensing of Carbonate Intercalation in Breast Microcalcifications at Stereotactic Biopsy.” Sci. Rep. 5 (April 30, 2015): 9907.
Version
Final published version
Abstract
Microcalcifications are an early mammographic sign of breast cancer and frequent target for stereotactic biopsy. Despite their indisputable value, microcalcifications, particularly of the type II variety that are comprised of calcium hydroxyapatite deposits, remain one of the least understood disease markers. Here we employed Raman spectroscopy to elucidate the relationship between pathogenicity of breast lesions in fresh biopsy cores and composition of type II microcalcifications. Using a chemometric model of chemical-morphological constituents, acquired Raman spectra were translated to characterize chemical makeup of the lesions. We find that increase in carbonate intercalation in the hydroxyapatite lattice can be reliably employed to differentiate benign from malignant lesions, with algorithms based only on carbonate and cytoplasmic protein content exhibiting excellent negative predictive value (93–98%). Our findings highlight the importance of calcium carbonate, an underrated constituent of microcalcifications, as a spectroscopic marker in breast pathology evaluation and pave the way for improved biopsy guidance.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Spectroscopy Laboratory
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/srep09907
