Responsive Microgrooves for the Formation of Harvestable Tissue Constructs
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Gleason_Responsive Microgrooves.pdf
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Author(s)
Tekin, Halil
Ozaydin-Ince, Gozde
Tsinman, Tonia
Khademhosseini, Ali
Demirel, Melik C.
Gleason, Karen K
Langer, Robert S
Date Issued
March 2011
Journal
Langmuir
Publisher
American Chemical Society
Citation
Tekin, Halil, Gozde Ozaydin-Ince, Tonia Tsinman, Karen K. Gleason, Robert Langer, Ali Khademhosseini, and Melik C. Demirel. Responsive Microgrooves for the Formation of Harvestable Tissue Constructs. Langmuir 27, no. 9: 5671-5679.
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Author's final manuscript
Abstract
Given its biocompatibility, elasticity, and gas permeability, poly(dimethylsiloxane) (PDMS) is widely used to fabricate microgrooves and microfluidic devices for three-dimensional (3D) cell culture studies. However, conformal coating of complex PDMS devices prepared by standard microfabrication techniques with desired chemical functionality is challenging. This study describes the conformal coating of PDMS microgrooves with poly(N-isopropylacrylamide) (PNIPAAm) by using initiated chemical vapor deposition (iCVD). These microgrooves guided the formation of tissue constructs from NIH-3T3 fibroblasts that could be retrieved by the temperature-dependent swelling property and hydrophilicity change of the PNIPAAm. The thickness of swollen PNIPAAm films at 24 °C was approximately 3 times greater than at 37 °C. Furthermore, PNIPAAm-coated microgroove surfaces exhibit increased hydrophilicity at 24 °C (contact angle θ = 30° ± 2) compared to 37 °C (θ = 50° ± 1). Thus PNIPAAm film on the microgrooves exhibits responsive swelling with higher hydrophilicity at room temperature, which could be used to retrieve tissue constructs. The resulting tissue constructs were the same size as the grooves and could be used as modules in tissue fabrication. Given its ability to form and retrieve cell aggregates and its integration with standard microfabrication, PNIPAAm-coated PDMS templates may become useful for 3D cell culture applications in tissue engineering and drug discovery.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1021/la200183x
