In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes
Name
Strano_In vivio biosensing.pdf
Size
923.43 KB
Format
Adobe PDF
Checksum (MD5)
be758f1485a2f1a09df899c985e32808
Author(s)
Iverson, Nicole M.
Barone, Paul W.
Shandell, Mia
Trudel, Laura J.
Sen, Selda
Sen, Fatih
Ivanov, Vsevolod
Atolia, Esha
Farias, Edgardo
McNicholas, Thomas P.
Date Issued
November 2013
Journal
Nature Nanotechnology
Publisher
Nature Publishing Group
Citation
Iverson, Nicole M., Paul W. Barone, Mia Shandell, Laura J. Trudel, Selda Sen, Fatih Sen, Vsevolod Ivanov, et al. “In Vivo Biosensing via Tissue-Localizable Near-Infrared-Fluorescent Single-Walled Carbon Nanotubes.” Nature Nanotechnology 8, no. 11 (November 3, 2013): 873–880.
Version
Author's final manuscript
Abstract
Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for various compounds, their use as in vivo biomarkers requires the simultaneous optimization of various parameters, including biocompatibility, molecular recognition, high fluorescence quantum efficiency and signal transduction. Here we show that a polyethylene glycol ligated copolymer stabilizes near-infrared-fluorescent single-walled carbon nanotubes sensors in solution, enabling intravenous injection into mice and the selective detection of local nitric oxide concentration with a detection limit of 1 µM. The half-life for liver retention is 4 h, with sensors clearing the lungs within 2 h after injection, thus avoiding a dominant route of in vivo nanotoxicology. After localization within the liver, it is possible to follow the transient inflammation using nitric oxide as a marker and signalling molecule. To this end, we also report a spatial-spectral imaging algorithm to deconvolute fluorescence intensity and spatial information from measurements. Finally, we demonstrate that alginate-encapsulated single-walled carbon nanotubes can function as implantable inflammation sensors for nitric oxide detection, with no intrinsic immune reactivity or other adverse response for more than 400 days.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Innovation
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nnano.2013.222
