Protein-targeted corona phase molecular recognition
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Bisker-2016-Protein-targeted.pdf
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Author(s)
Iverson, Nicole M.
Ahn, Jiyoung
Nelson, Justin Theodore
Landry, Markita P.
Kruss, Sebastian
Strano, Michael S.
Bisker Raviv, Gili Hana
Dong, Juyao-Ivy
Park, Hoyoung Daniel
Date Issued
January 2016
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Bisker, Gili, Juyao Dong, Hoyoung D. Park, Nicole M. Iverson, Jiyoung Ahn, Justin T. Nelson, Markita P. Landry, Sebastian Kruss, and Michael S. Strano. “Protein-Targeted Corona Phase Molecular Recognition.” Nat Comms 7 (January 8, 2016): 10241.
Version
Final published version
Abstract
Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1038/ncomms10241
