CENTRIFUGAL LABTUBE FOR FULLY AUTOMATED DNA EXTRACTION & LAMP AMPLIFICATION BASED ON AN INTEGRATED, LOW-COST HEATING SYSTEM
Name
uTAS_heatedLabTube_finalpaper.pdf
Size
581.88 KB
Format
Adobe PDF
Checksum (MD5)
01a5267ce43d6c50c0f26469e3578a2f
Author(s)
Hoehl, Melanie Margarete
Weibert, Michael
Paust, Nils
Zengerle, Roland
Slocum, Alexander H.
Steigert, Juergen
Date Issued
October 2013
Journal
Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
Publisher
Transducers Research Foundations, Inc.
Citation
Hoehl, Melanie, Michael Weißert, Nils Paust, Roland Zengerle, et al. "CENTRIFUGAL LABTUBE FOR FULLY AUTOMATED DNA EXTRACTION & LAMP AMPLIFICATION BASED ON AN INTEGRATED, LOW-COST HEATING SYSTEM. 'In the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), Freiburg, Germany, 27-31 October 2013.
Version
Author's final manuscript
Abstract
In this paper, we introduce a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA-extraction platform (LabTube). We demonstrate fully automated, fully closed extraction of as little as 100 DNA copies of verotoxin-producing (VTEC) Escherichia coli lysate in water, milk and apple juice in a standard laboratory centrifuge, followed by subsequent automatic LAMP amplification with an overall time-to-result of 1.5hrs. The system is disposable, fully closed and automated, requiring only a single pipetting step. The microcontroller-driven heating system is low-cost (<1$) and it can be easily parallelized. Because the heated LabSystem runs within a standard laboratory centrifuge, it is suitable for DNA extraction and amplification in low-resource areas, at production sites or sales locations.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
http://www.microtas2013.org/program/MicroTAS2013_TechnicalProgram.pdf
