Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests
Name
nihms799182.pdf
Description
Accepted version
Size
1.46 MB
Format
Unknown
Checksum (MD5)
8008a021328f7c271960de2452900f20
Author(s)
Miller, E. A.
Traxlmayr, Michael
Shen, Julie
Sikes, Hadley D.
Date Issued
2016
Journal
Molecular Systems Design and Engineering
Publisher
Royal Society of Chemistry (RSC)
Citation
Miller, E. A., et al. "Activity-Based Assessment of an Engineered Hyperthermophilic Protein as a Capture Agent in Paper-Based Diagnostic Tests." Mol Syst Des Eng 1 4 (2016): 377-81.
Version
Author's final manuscript
Abstract
This journal is © The Royal Society of Chemistry. Antibodies have traditionally served as the affinity reagents of choice in point-of-care diagnostic biosensors. However, this class of proteins is not ideally suited for this use, being poorly characterized and prone to thermal denaturation. Here, we present an activity-based assessment of an alternative engineered binding protein in a cellulose-based assay.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/C6ME00032K
