Genetic requirements for cell division in a genomically minimal cell
Name
2020.10.07.326892v1.full.pdf
Description
Submitted version
Size
13.68 MB
Format
Adobe PDF
Checksum (MD5)
0cc35d63c2fbfa3af726b9da95c24c38
Author(s)
Pelletier, James F
Sun, Lijie
Wise, Kim S
Assad-Garcia, Nacyra
Karas, Bogumil J
Deerinck, Thomas J
Ellisman, Mark H
Mershin, Andreas
Gershenfeld, Neil
Chuang, Ray-Yuan
Date Issued
2021
Journal
Cell
Publisher
Elsevier BV
Citation
Pelletier, James F, Sun, Lijie, Wise, Kim S, Assad-Garcia, Nacyra, Karas, Bogumil J et al. 2021. "Genetic requirements for cell division in a genomically minimal cell." Cell, 184 (9).
Version
Original manuscript
Abstract
Genomically minimal cells, such as JCVI-syn3.0, offer a platform to clarify genes underlying core physiological processes. Although this minimal cell includes genes essential for population growth, the physiology of its single cells remained uncharacterized. To investigate striking morphological variation in JCVI-syn3.0 cells, we present an approach to characterize cell propagation and determine genes affecting cell morphology. Microfluidic chemostats allowed observation of intrinsic cell dynamics that result in irregular morphologies. A genome with 19 genes not retained in JCVI-syn3.0 generated JCVI-syn3A, which presents morphology similar to that of JCVI-syn1.0. We further identified seven of these 19 genes, including two known cell division genes, ftsZ and sepF, a hydrolase of unknown substrate, and four genes that encode membrane-associated proteins of unknown function, which are required together to restore a phenotype similar to that of JCVI-syn1.0. This result emphasizes the polygenic nature of cell division and morphology in a genomically minimal cell.
MIT Department
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.CELL.2021.03.008
