Methods and models of screening genomic variants
Name
frangieh-frangieh-phd-eecs-2023-thesis.pdf
Description
Thesis PDF
Size
13.82 MB
Format
Adobe PDF
Checksum (MD5)
33d1f78ebe67bc9d9b73c4a818563687
Author(s)
Frangieh, Chris J.
Advisor(s)
Zhang, Feng
Regev, Aviv
Date Issued
September 2023
Publisher
Massachusetts Institute of Technology
Abstract
Genomes are the basis of human biology and human disease. Understanding the role of each gene on a healthy or diseased phenotype requires an intervention to causally link between genotype and phenotype. Advances in RNA-guided endonucleases have enabled such pooled screens in human cells. I first consider a model to understand drivers of immune evasion in a pooled knockout screen conducted in an in vitro model of metastatic melanoma. Next, I discuss strategies for scaling these screens to encompass a larger set of genes from the human genome. Finally, I explore how next-generation genome editors can move beyond knockout screens to identify the biological role of any sequence at any location in the human genome.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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