A Rheological Study of the Association and Dynamics of MUC5AC Gels
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Author(s)
Rubinstein, Michael
Wagner, Caroline E
Turner, Bradley S.
McKinley, Gareth H
Ribbeck, Katharina
Date Issued
November 2017
Journal
Biomacromolecules
Publisher
American Chemical Society (ACS)
Citation
Wagner, Caroline E. et al. “A Rheological Study of the Association and Dynamics of MUC5AC Gels.” Biomacromolecules 18, 11 (October 2017): 3654–3664 © 2017 American Chemical Society
Version
Author's final manuscript
Abstract
The details of how a mucus hydrogel forms from its primary structural component, mucin polymers, remain incompletely resolved. To explore this, we use a combination of macrorheology and single-particle tracking to investigate the bulk and microscopic mechanical properties of reconstituted MUC5AC mucin gels. We find that analyses of thermal fluctuations on the length scale of the micrometer-sized particles are not predictive of the linear viscoelastic response of the mucin gels, and that taken together, the results from both techniques help to provide complementary insight into the structure of the network. In particular, we show that macroscopic stiffening of MUC5AC gels can be brought about in different ways by targeting specific associations within the network using environmental triggers such as modifications to the pH, surfactant, and salt concentration. Our work may be important for understanding how environmental factors, including pathogens and therapeutic agents, alter the mechanical properties of fully constituted mucus.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ACS.BIOMAC.7B00809
