Engineered plant control of associative nitrogen fixation
Name
pnas.2117465119.pdf
Description
Published version
Size
1.43 MB
Format
Adobe PDF
Checksum (MD5)
b3ad27f258eca955adaa6205eebf1bd7
Author(s)
Haskett, Timothy L
Paramasivan, Ponraj
Mendes, Marta D
Green, Patrick
Geddes, Barney A
Knights, Hayley E
Jorrin, Beatriz
Ryu, Min-Hyung
Brett, Paul
Voigt, Christopher A
Date Issued
2022
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
Proceedings of the National Academy of Sciences
Citation
Haskett, Timothy L, Paramasivan, Ponraj, Mendes, Marta D, Green, Patrick, Geddes, Barney A et al. 2022. "Engineered plant control of associative nitrogen fixation." Proceedings of the National Academy of Sciences of the United States of America, 119 (16).
Version
Final published version
Abstract
Significance
Inoculation of cereals with diazotrophic (N 2 -fixing) bacteria offers a sustainable alternative to the application of nitrogen fertilizers in agriculture. While natural diazotrophs have evolved multilayered regulatory mechanisms that couple N 2 fixation with assimilation of the product NH 3 and prevent release to plants, genetic modifications can permit excess production and excretion of NH 3 . However, a lack of stringent host-specificity for root colonization by the bacteria would allow growth promotion of target and nontarget plants species alike. Here, we exploit synthetic transkingdom signaling to establish plant host-specific control of the N 2 -fixation catalyst nitrogenase in Azorhizobium caulinodans occupying barley roots. This work demonstrates how partner-specific interactions can be established to avoid potential growth promotion of nontarget plants.
Inoculation of cereals with diazotrophic (N 2 -fixing) bacteria offers a sustainable alternative to the application of nitrogen fertilizers in agriculture. While natural diazotrophs have evolved multilayered regulatory mechanisms that couple N 2 fixation with assimilation of the product NH 3 and prevent release to plants, genetic modifications can permit excess production and excretion of NH 3 . However, a lack of stringent host-specificity for root colonization by the bacteria would allow growth promotion of target and nontarget plants species alike. Here, we exploit synthetic transkingdom signaling to establish plant host-specific control of the N 2 -fixation catalyst nitrogenase in Azorhizobium caulinodans occupying barley roots. This work demonstrates how partner-specific interactions can be established to avoid potential growth promotion of nontarget plants.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1073/PNAS.2117465119
