Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution
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Lander_Genome-scale.pdf
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Author(s)
Gu, Hongcang
Bock, Christoph
Jäger, Natalie
Smith, Zachary D.
Tomazou, Eleni
Gnirke, Andreas
Meissner, Alexander
Mikkelsen, Tarjei Sigurd, 1978-
Lander, Eric Steven
Date Issued
January 2010
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Gu, Hongcang et al. “Genome-scale DNA Methylation Mapping of Clinical Samples at Single-nucleotide Resolution.” Nature Methods 7.2 (2010): 133–136.
Version
Author's final manuscript
Abstract
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution,
providing a robust platform for molecular diagnostics. Here, we optimize bisulfite sequencing for
genome-scale analysis of clinical samples. Specifically, we outline how restriction digestion
targets bisulfite sequencing to hotspots of epigenetic regulation; we show that 30ng of DNA are
sufficient for genome-scale analysis; we demonstrate that our protocol works well on formalinfixed,
paraffin-embedded (FFPE) samples; and we describe a statistical method for assessing
significance of altered DNA methylation patterns.
Description
August 1, 2010
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1038/nmeth.1414
