Prion Protein is Expressed on Long-term Repopulating Hematopoietic Stem Cells and is Necessary for their Self-renewal
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MEBCS017.pdf
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Author(s)
Lodish, Harvey F.
Zhang, Cheng Cheng
Steele, Andrew D.
Lindquist, Susan L.
Date Issued
January 2005
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS);
Abstract
We show that the prion protein (PrP) is expressed on the surface of bone marrow cell populations enriched in long-term repopulating hematopoietic stem cells. Affinity purification of the PrP-positive and PrP-negative fractions from these populations, followed by competitive reconstitution assays, show that all long-term repopulating hematopoietic stem cells express PrP. Hematopoietic stem cells from PrP null bone marrow exhibit impaired self-renewal in serial competitive transplantation experiments, and premature exhaustion when exposed to cell cycle-specific myelotoxic injury. Therefore, PrP is a novel marker for hematopoietic stem cells and regulates their self-renewal.
Subjects
Prion Proteins
PrP
hematopoietic stem cells
bone marrow cells
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