7.344 Directed Evolution: Engineering Biocatalysts, Spring 2008
Author(s)
Love, Kerry
Alternative Title
Directed Evolution: Engineering Biocatalysts
Date Issued
June 2008
Abstract
Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching.
Subjects
evolution
biocatalyst
mutation
library
recombination
directed evolution
enzyme
point mutation
mutagenesis
DNA
evolution
gene
complementation
affinity
phage
ribosome display
yeast surface display
bacterial cell surface display
IVC
FACS
active site
MIT Department
Massachusetts Institute of Technology. Department of Biology
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