Implantable microenvironments to attract hematopoietic stem/cancer cells
Name
Lee-2012-Implantable microenv.pdf
Size
1.41 MB
Format
Adobe PDF
Checksum (MD5)
2405e51eb5092858e381ddc2e961b6d5
Author(s)
Lee, Jungwoo
Li, Matthew
Milwid, Jack
Dunham, Joshua
Vinegoni, Claudio
Gorbatov, Rostic
Iwamoto, Yoshiko
Wang, Fangjing
Shen, Keyue
Hatfield, Kimberley
Date Issued
November 2012
Journal
Proceedings of the National Academy of Sciences
Publisher
Public Library of Science
Citation
Lee, J. et al. “Implantable Microenvironments to Attract Hematopoietic Stem/cancer Cells.” Proceedings of the National Academy of Sciences 109.48 (2012): 19638–19643.
Version
Final published version
Abstract
The environments that harbor hematopoietic stem and progenitor cells are critical to explore for a better understanding of hematopoiesis during health and disease. These compartments often are inaccessible for controlled and rapid experimentation, thus limiting studies to the evaluation of conventional cell culture and transgenic animal models. Here we describe the manufacture and image-guided monitoring of an engineered microenvironment with user-defined properties that recruits hematopoietic progenitors into the implant. Using intravital imaging and fluorescence molecular tomography, we show in real time that the cell homing and retention process is efficient and durable for short- and long-term engraftment studies. Our results indicate that bone marrow stromal cells, precoated on the implant, accelerate the formation of new sinusoidal blood vessels with vascular integrity at the microcapillary level that enhances the recruitment hematopoietic progenitor cells to the site. This implantable construct can serve as a tool enabling the study of hematopoiesis.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1208384109
