Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome
Name
13287_2018_Article_982.pdf
Size
1.87 MB
Format
Adobe PDF
Checksum (MD5)
20e7561984cc0e785e78ee0e1f6c144f
Author(s)
Tam, Kimberley
Poon, Zhiyong
Van Vliet, Krystyn J
Liu, Frances D.
Liu, Frances Deen
Pishesha, Novalia
Date Issued
October 2018
Journal
Stem Cell Research & Therapy
Publisher
BioMed Central
Citation
Liu, Frances D. et al. "Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome." Stem Cell Research & Therapy 2018, 9 (October 2018): 268 © 2018 The Author(s)
Version
Final published version
Abstract
Background
Efficient and sustained hematopoietic recovery after hematopoietic stem cell or bone marrow transplantation is supported by paracrine signaling from specific subpopulations of mesenchymal stromal cells (MSCs). Here, we considered whether in vitro mechanopriming of human MSCs could be administered to predictively and significantly improve in vivo hematopoietic recovery after irradiation injury.
Methods
First, we implemented regression modeling to identify eight MSC-secreted proteins that correlated strongly with improved rescue from radiation damage, including hematopoietic recovery, in a murine model of hematopoietic failure. Using these partial least squares regression (PLSR) model parameters, we then predicted recovery potential of MSC populations that were culture expanded on substrata of varying mechanical stiffness. Lastly, we experimentally validated these predictions using an in vitro co-culture model of hematopoiesis and using new in vivo experiments for the same irradiation injury model used to generate survival predictions.
Results
MSCs grown on the least stiff (elastic moduli ~ 1 kPa) of these polydimethylsiloxane (PDMS) substrata secreted high concentrations of key proteins identified in regression modeling, at concentrations comparable to those secreted by minor subpopulations of MSCs shown previously to be effective in supporting such radiation rescue. We confirmed that these MSCs expanded on PDMS could promote hematopoiesis in an in vitro co-culture model with hematopoietic stem and progenitor cells (HSPCs). Further, MSCs cultured on PDMS of highest stiffness (elastic moduli ~ 100 kPa) promoted expression of CD123+ HSPCs, indicative of myeloid differentiation. Systemic administration of mechanoprimed MSCs resulted in improved mouse survival and weight recovery after bone marrow ablation, as compared with both standard MSC expansion on stiffer materials and with biophysically sorted MSC subpopulations. Additionally, we observed recovery of white blood cells, platelets, and red blood cells, indicative of complete recovery of all hematopoietic lineages.
Conclusions
These results demonstrate that computational techniques to identify MSC biomarkers can be leveraged to predict and engineer therapeutically effective MSC phenotypes defined by mechanoprimed secreted factors, for translational applications including hematopoietic recovery. Keywords: mesenchymal stromal cells; secretome; mechanobiology; hematopoietic recovery; radiation injury
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/s13287-018-0982-2
