Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry
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nihms964513.pdf
Description
Accepted version
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3.3 MB
Format
Adobe PDF
Checksum (MD5)
04a1cde2c34587b2b577dd03009cb8bd
Author(s)
Lalanne, Jean-Benoît
Taggart, James C.
Guo, Monica S.
Herzel, Lydia
Schieler, Ariel
Li, Gene-Wei
Date Issued
April 2018
Journal
Cell
Publisher
Elsevier BV
Citation
Lalanne, Jean-Benoît et al. “Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry.” Cell 173 (2018): 749-761.e38 © 2018 The Author(s)
Version
Author's final manuscript
Abstract
oexpression of proteins in response to pathway-inducing signals is the founding paradigm of gene regulation. However, it remains unexplored whether the relative abundance of co-regulated proteins requires precise tuning. Here, we present large-scale analyses of protein stoichiometry and corresponding regulatory strategies for 21 pathways and 67–224 operons in divergent bacteria separated by 0.6–2 billion years. Using end-enriched RNA-sequencing (Rend-seq) with single-nucleotide resolution, we found that many bacterial gene clusters encoding conserved pathways have undergone massive divergence in transcript abundance and architectures via remodeling of internal promoters and terminators. Remarkably, these evolutionary changes are compensated post-transcriptionally to maintain preferred stoichiometry of protein synthesis rates. Even more strikingly, in eukaryotic budding yeast, functionally analogous proteins that arose independently from bacterial counterparts also evolved to convergent in-pathway expression. The broad requirement for exact protein stoichiometries despite regulatory divergence provides an unexpected principle for building biological pathways both in nature and for synthetic activities.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.CELL.2018.03.007
