Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy
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Lau-2009-Re-evaluation of mod.pdf
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Author(s)
Lau, Condon
Scepanovic, Obrad R.
Markovic, Jelena
McGee, Sasha
Yu, Chung-Chieh
Fulghum, Stephen, Jr.
Wallace, Michael
Tunnell, James
Bechtel, Kate L.
Feld, Michael S.
Date Issued
April 2009
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-Optical Instrumentation Engineers
Citation
Lau, Condon et al. “Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy.” Journal of Biomedical Optics 14.2 (2009): 024031-8. © 2009 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Model-based light scattering spectroscopy (LSS) seemed a promising technique for in-vivo diagnosis of dysplasia in multiple organs. In the studies, the residual spectrum, the difference between the observed and modeled diffuse reflectance spectra, was attributed to single elastic light scattering from epithelial nuclei, and diagnostic information due to nuclear changes was extracted from it. We show that this picture is incorrect. The actual single scattering signal arising from epithelial nuclei is much smaller than the previously computed residual spectrum, and does not have the wavelength dependence characteristic of Mie scattering. Rather, the residual spectrum largely arises from assuming a uniform hemoglobin distribution. In fact, hemoglobin is packaged in blood vessels, which alters the reflectance. When we include vessel packaging, which accounts for an inhomogeneous hemoglobin distribution, in the diffuse reflectance model, the reflectance is modeled more accurately, greatly reducing the amplitude of the residual spectrum. These findings are verified via numerical estimates based on light propagation and Mie theory, tissue phantom experiments, and analysis of published data measured from Barrett's esophagus. In future studies, vessel packaging should be included in the model of diffuse reflectance and use of model-based LSS should be discontinued.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
http://dx.doi.org/10.1117/1.3116708
