Exploration of methods for many-site genome editing with MAGE
Name
778070056-MIT.pdf
Description
Full printable version
Size
2.61 MB
Format
Adobe PDF
Checksum (MD5)
6e29a0483c2edaf798743e5d2cb504e4
Author(s)
Sterling, Bram (Bram H.)
Advisor(s)
Joseph Jacobson.
Date Issued
2011
Publisher
Massachusetts Institute of Technology
Abstract
In order to modify bacterial genetic codes, modifications must be made throughout the genome wherever the affected codon is used. Making such numerous and widespread genomic changes requires specialized techniques. MAGE is well-suited to this task, being highly amenable to multiplexing and having low time and resource costs per site. MAGE has been used as a first stage in recoding efforts, converting small clusters of sites in separate strains to be combined by other means, but improvements in MAGE technique suggest the possibility of using it to produce fully-recoded strains directly. To this end, I compare strategies based on co-selected MAGE and apply the best by performing 80 site conversions spread over 1/4 of the E coli genome.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2011.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 28).
Subjects
Architecture. Program in Media Arts and Sciences.
MIT Department
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
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