Toward engineered processes for sequencing-based analysis of single circulating tumor cells
Name
nihms573283.pdf
Description
Accepted version
Size
518.79 KB
Format
Adobe PDF
Checksum (MD5)
2840d0d7fd86d341a27336cda52ec913
Author(s)
Adalsteinsson, Viktor A
Love, J Christopher
Date Issued
2014
Journal
Current Opinion in Chemical Engineering
Publisher
Elsevier BV
Citation
Adalsteinsson, Viktor A., and J. Christopher Love. "Toward Engineered Processes for Sequencing-Based Analysis of Single Circulating Tumor Cells." Current Opinion in Chemical Engineering 4 (2014): 97-104.
Version
Author's final manuscript
Abstract
Sequencing-based analysis of single circulating tumor cells (CTCs) has the potential to revolutionize our understanding of metastatic cancer and improve clinical care. Technologies exist to enrich, identify, recover, and sequence single cells, but to enable systematic routine analysis of single CTCs from a range of cancer patients, there is a need to establish processes that efficiently integrate these specific operations. Such engineered processes should address challenges associated with the yield and viability of enriched CTCs, the robust identification of candidate single CTCs with minimal degradation of DNA, the bias in whole-genome amplification, and the efficient handling of candidate single CTCs or their amplified DNA products. Advances in methods for single-cell analysis and nanoscale technologies suggest opportunities to overcome these challenges, and could create integrated platforms that perform several of the unit operations together. Ultimately, technologies should be selected or adapted for optimal performance and compatibility in an integrated process. © 2014 Elsevier Ltd.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.COCHE.2014.01.011
