Biased chromatin signatures around polyadenylation sites and exons
Name
Burge_Biased Chromatin.pdf
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1.32 MB
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Adobe PDF
Checksum (MD5)
89882d935774f6e1b525c235935faf78
Author(s)
Nielsen, Cydney B.
Padgett, Richard A.
Spies, Noah
Burge, Christopher B
Date Issued
October 2009
Journal
Molecular Cell
Publisher
Elsevier
Citation
Spies, Noah et al. “Biased Chromatin Signatures Around Polyadenylation Sites and Exons.” Molecular Cell 36.2 (2009): 245–254.
Version
Author's final manuscript
Abstract
Core RNA-processing reactions in eukaryotic cells occur cotranscriptionally in a chromatin context, but the relationship between chromatin structure and pre-mRNA processing is poorly understood. We observed strong nucleosome depletion around human polyadenylation sites (PAS) and nucleosome enrichment just downstream of PAS. In genes with multiple alternative PAS, higher downstream nucleosome affinity was associated with higher PAS usage, independently of known PAS motifs that function at the RNA level. Conversely, exons were associated with distinct peaks in nucleosome density. Exons flanked by long introns or weak splice sites exhibited stronger nucleosome enrichment, and incorporation of nucleosome density data improved splicing simulation accuracy. Certain histone modifications, including H3K36me3 and H3K27me2, were specifically enriched on exons, suggesting active marking of exon locations at the chromatin level. Together, these findings provide evidence for extensive functional connections between chromatin structure and RNA processing.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2009.10.008
