A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production
Name
Love_A perfusion-capable.pdf
Size
1.17 MB
Format
Adobe PDF
Checksum (MD5)
378e2dc400fbcac5cdfa1958c40c81d0
Author(s)
Lee, Kevin S.
Lee, Harry L. T.
Mozdzierz, Nicholas Joseph
Love, Kerry R.
Shah, Kartik A
Ram, Rajeev J
Love, John C
Date Issued
May 2015
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry, The
Citation
Mozdzierz, Nicholas J.; Love, Kerry R.; Lee, Kevin S.; Lee, Harry L. T.; Shah, Kartik A.; Ram, Rajeev J. and Love, J. Christopher. “A Perfusion-Capable Microfluidic Bioreactor for Assessing Microbial Heterologous Protein Production.” Lab on a Chip 15, no. 14 (May 2015): 2918–2922 © 2015 Royal Society of Chemistry
Version
Author's final manuscript
Abstract
We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Innovation
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Research Laboratory of Electronics
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/c5lc00443h
