Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes
Name
Bader-2016-Grown, Printed, and.pdf
Size
554.61 KB
Format
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Checksum (MD5)
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Author(s)
Hays, Stephanie G.
Dikovsky, Daniel
Belocon, Boris
Weaver, James C.
Silver, Pamela A.
Bader, Christoph
Patrick, William Graham
Kolb, Dominik
Keating, Steven John
Sharma, Sunanda
Date Issued
June 2016
Journal
3D Printing and Additive Manufacturing
Publisher
Mary Ann Liebert, Inc.
Citation
Bader, Christoph; Patrick, William G.; Kolb, Dominik; Hays, Stephanie G.; Keating, Steven; Sharma, Sunanda; Dikovsky, Daniel et al. “Grown, Printed, and Biologically Augmented: An Additively Manufactured Microfluidic Wearable, Functionally Templated for Synthetic Microbes.” 3D Printing and Additive Manufacturing 3, no. 2 (June 2016): 79–89 © 2016 Mary Ann Liebert, Inc
Version
Final published version
Abstract
Despite significant advances in synthetic biology at industrial scales, digital fabrication challenges have, to date, precluded its implementation at the product scale. We present, Mushtari, a multimaterial 3D printed fluidic wearable designed to culture microbial communities. Thereby we introduce a computational design environment for additive manufacturing of geometrically complex and materially heterogeneous fluidic channels. We demonstrate how controlled variation of geometrical and optical properties at high spatial resolution can be achieved through a combination of computational growth modeling and multimaterial bitmap printing. Furthermore, we present the implementation, characterization, and evaluation of support methods for creating product-scale fluidics. Finally, we explore the cytotoxicity of 3D printed materials in culture studies with the model microorganisms, Escherichia coli and Bacillus subtilis. The results point toward design possibilities that lie at the intersection of computational design, additive manufacturing, and synthetic biology, with the ultimate goal of imparting biological functionality to 3D printed products.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1089/3dp.2016.0027
