Risk Assessment of Synthetic Genomics: A Biosafety and Biosecurity Perspective
Name
Fleming Biosafety Biosecurity.pdf
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339.7 KB
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Adobe PDF
Checksum (MD5)
aa8f62f23aaeb754a652e0b7008a2d6b
Author(s)
Fleming, Diane
Date Issued
December 4, 2007
Abstract
The ability to synthesize molecules found in living organisms is not new for scientists in
the fields of biochemistry and molecular biology. However, the “synthetic biology” made
possible by the genetic mapping of microorganisms, plants and animals, including the
human genome, has taken this area of science into new and relatively uncharted territory.
The focus here will be on “synthetic genomics” in which genetic information is
synthesized using chemical components and the genomic DNA sequence of an organism.
This is how investigators at the State University of New York in Stony Brook, using a
published genetic sequence, synthesized a DNA version of poliovirus in 2002. Using an
enzyme, reverse transcriptase, they converted the DNA to RNA and were able to grow
the virus in a cell-free extract. Their synthesized poliovirus caused paralysis in animals
(Cello et al., 2002). One of the authors, Eckard Wimmer, warned: “The world had better
be prepared. This shows you can re-create a virus from written information.”
From a biosafety and biosecurity perspective the synthesis of etiologic agents is of
concern because of the potential to create completely new combinations or chimeric
genomes with enhanced virulence, extended host range, and resistance to antimicrobials,
antivirals or vaccines. A major concern is that an agent which has been eradicated as a
source of infectious disease, such as smallpox, and one which is in the process of being
eradicated, such as poliovirus, will never be truly eliminated because the information for
their synthesis is readily available in sequence databases.
Description
Technical report in support of the Sloan Foundation funded study on DNA synthesis and governance.
Subjects
synthetic biology
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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