A pathway for mitotic chromosome formation
Name
nihms956518.pdf
Description
Accepted version
Size
2.74 MB
Format
Adobe PDF
Checksum (MD5)
18c1e9afa468ab3d769145b229fc4e37
Author(s)
Gibcus, Johan H.
Samejima, Kumiko
Goloborodko, Anton
Samejima, Itaru
Naumova, Natalia
Nubler, Johannes
Kanemaki, Masato T.
Xie, Linfeng
Paulson, James R.
Earnshaw, William C.
Date Issued
January 2018
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Gibcus, Johan H. et al. "A pathway for mitotic chromosome formation." Science 359, 6376 (January 2018): eaao6135 © 2017 The Authors
Version
Author's final manuscript
Abstract
Mitotic chromosomes fold as compact arrays of chromatin loops.To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed.During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops.The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold.The size of helical turns progressively increases to ~12megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/science.aao6135
