Sinorhizobium meliloti CpdR1 is critical for coordinating cell-cycle progression and the symbiotic chronic infection
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Walker_Sinorhizobium meliloti.pdf
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Author(s)
Kobayashi, Hajime
De Nisco, Nicole J.
Chien, Peter
Simmons, Lyle A.
Walker, Graham C.
Date Issued
July 2009
Journal
Molecular Microbiology
Publisher
Wiley Blackwell
Citation
Kobayashi, Hajime et al. “Sinorhizobium Meliloti CpdR1 Is Critical for Co-ordinating Cell Cycle Progression and the Symbiotic Chronic Infection.” Molecular Microbiology 73.4 (2009): 586–600.
Version
Author's final manuscript
Abstract
ATP-driven proteolysis plays a major role in regulating the bacterial cell cycle, development and stress responses. In the nitro -fixing symbiosis with host plants, Sinorhizobium meliloti undergoes a profound cellular differentiation, including endoreduplication of the ome. The regulatory mechanisms governing the alterations of the S. meliloti cell cycle in planta are largely unknown. Here, we report the characterization of two cpdR homologues, cpdR1 and cpdR2, of S. meliloti that encode single-domain response regulators. In Caulobacter crescentus, CpdR controls the polar localization of the ClpXP protease, thereby mediating the regulated proteolysis of key protein(s), such as CtrA, involved in cell cycle progression. The S. meliloti cpdR1-null mutant can invade the host cytoplasm, however, the intracellular bacteria are unable to differentiate into bacteroids. We show that S. meliloti CpdR1 has a polar localization pattern and a role in ClpX positioning similar to C. crescentus CpdR, suggesting a conserved function of CpdR proteins among α-proteobacteria. However, in S. meliloti, free-living cells of the cpdR1-null mutant show a striking morphology of irregular coccoids and aberrant DNA replication. Thus, we demonstrate that CpdR1 mediates the co-ordination of cell cycle events, which are critical for both the free-living cell division and the differentiation required for the chronic intracellular infection.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1111/j.1365-2958.2009.06794.x
