Intracellular sensor spatial multiplexing via RNA scaffolds
Name
1418760885-MIT.pdf
Size
3.7 MB
Format
Adobe PDF
Checksum (MD5)
4351256d812fccc0d574ba4f78a3119b
Author(s)
Johnson, Shannon L.
Advisor(s)
Edward S. Boyden.
Date Issued
2019
Publisher
Massachusetts Institute of Technology
Abstract
To circumvent the limitations of spectrally multiplexing sensors, fluorescent sensors are clustered by type and spatially separated in the cytoplasm to avoid cross-talk. Each sensor is fused to an orthogonal viral capsid protein that binds to a long, repetitive strand of its corresponding RNA sequence. All sensors fluoresce green and are indistinguishable during recording but are identified with post-hoc antibody or FISH staining for each sensor-specific puncta. This spatial multiplexing strategy will allow for easier scaling of the number of fluorescent reporters of physiological activity.
Description
Thesis: S.M., Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2019
Cataloged from the official PDF version of thesis.
Includes bibliographical references (pages 38-40).
Subjects
Program in Media Arts and Sciences
MIT Department
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.
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