Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells
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Roles of H3K27me2.pdf
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4.01 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Juan, Aster H.
Wang, Stan
Ko, Kyung Dae
Zare, Hossein
Tsai, Pei-Fang
Feng, Xuesong
Ascoli, Anthony M.
Gutierrez-Cruz, Gustavo
Krebs, Jordan
Sidoli, Simone
Date Issued
October 2016
Journal
Cell Reports
Publisher
Elsevier
Citation
Juan, Aster H. et al. “Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells.” Cell Reports 17.5 (2016): 1369–1382. © 2017 Elsevier
Version
Final published version
Abstract
The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2016.09.087
