An evaluation of cytokine-capture nanoparticle technology : stepping from bench-space into potential markets
Name
60425195-MIT.pdf
Description
Full printable version
Size
6.31 MB
Format
Adobe PDF
Checksum (MD5)
ded4341136864512b5728e56104cf6f7
Author(s)
Hong, Julee Y. (Julee Yang-A.), 1980-
Advisor(s)
Darrell J. Irvine.
Date Issued
2004
Publisher
Massachusetts Institute of Technology
Abstract
The feasibility of bringing a nascent technology for detection and quantification of local cytokine concentrations on cell surfaces to market is presented in this paper. Quantum dots or fluorochrome-loaded nanoparticles are conjugated with antibodies for target analytes and with proteins that allow nanoparticle attachment to the surface of T cells. A second labeled monoclonal antibody is introduced to detect the presence of any captured-cytokines using 3D fluorescent microscopy or flow cytometry. Microscopy of DO.ll cells labeled with cytokine-capture particles have shown successful detection of exogenous IL2. A comparison of existing patents with cytokine-capture technology revealed that although each aspect of the device is covered by prior IP, the capabilities of the technology exceed the claimed uses of the individual components. A preliminary market research for cytokine-capture technology applications resulted in dismissing the immunoassay industry as a target market. However, T cell monitoring was identified as a far more lucrative industry.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2004.
Includes bibliographical references (leaves 65-66).
Subjects
Materials Science and Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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