Constructing an adult orofacial premotor atlas in Allen mouse CCF
Name
elife-67291-v2.pdf
Description
Published version
Size
34.84 MB
Format
Unknown
Checksum (MD5)
8f0ff8548ca7ea11584447552c93963a
Author(s)
Takatoh, Jun
Park, Jae Hong
Lu, Jinghao
Li, Shun
Thompson, PM
Han, Bao-Xia
Zhao, Shengli
Kleinfeld, David
Friedman, Beth
Wang, Fan
Date Issued
April 2021
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Takatoh, Jun, Park, Jae Hong, Lu, Jinghao, Li, Shun, Thompson, PM et al. 2021. "Constructing an adult orofacial premotor atlas in Allen mouse CCF." eLife, 10.
Version
Final published version
Abstract
Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/elife.67291
