Electrochemical Strategy for Low-Cost Viral Detection
Name
zamani-et-al-2021-electrochemical-strategy-for-low-cost-viral-detection.pdf
Description
Published version
Size
6.2 MB
Format
Adobe PDF
Checksum (MD5)
f83d539518780dcc31d18fcc922e1e0b
Author(s)
Zamani, Marjon
Robson, James M
Fan, Andy
Bono, Michael S
Furst, Ariel L
Klapperich, Catherine M
Date Issued
June 23, 2021
Journal
ACS Central Science
Publisher
American Chemical Society
Citation
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono Jr., Ariel L. Furst, and Catherine M. Klapperich. ACS Central Science 2021 7 (6), 963-972.
Version
Final published version
Abstract
Sexually transmitted infections, including the human immunodeficiency virus (HIV) and the human papillomavirus (HPV), disproportionally impact those in low-resource settings. Early diagnosis is essential for managing HIV. Similarly, HPV causes nearly all cases of cervical cancer, the majority (90%) of which occur in low-resource settings. Importantly, infection with HPV is six times more likely to progress to cervical cancer in women who are HIV-positive. An inexpensive, adaptable point-of-care test for viral infections would make screening for these viruses more accessible to a broader set of the population. Here, we report a novel, cost-effective electrochemical platform using gold leaf electrodes to detect clinically relevant viral loads. We have combined this platform with loop-mediated isothermal amplification and a CRISPR-based recognition assay to detect HPV. Lower limits of detection were demonstrated down to 104 total copies of input nucleic acids, which is a clinically relevant viral load for HPV DNA. Further, proof-of-concept experiments with cervical swab samples, extracted using standard extraction protocols, demonstrated that the strategy is extendable to complex human samples. This adaptable technology could be applied to detect any viral infection rapidly and cost-effectively.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/acscentsci.1c00186
