Rule-Based Design of Synthetic Transcription Factors in Eukaryotes
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Lu_Rule-based design.pdf
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282.99 KB
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Author(s)
Purcell, Oliver
Peccoud, Jean
Lu, Timothy K.
Date Issued
December 2013
Journal
ACS Synthetic Biology
Publisher
American Chemical Society (ACS)
Citation
Purcell, Oliver, Jean Peccoud, and Timothy K. Lu. “Rule-Based Design of Synthetic Transcription Factors in Eukaryotes.” ACS Synthetic Biology 3, no. 10 (October 17, 2014): 737–744.
Version
Author's final manuscript
Abstract
To design and build living systems, synthetic biologists have at their disposal an increasingly large library of naturally derived and synthetic parts. These parts must be combined together in particular orders, orientations, and spacings to achieve desired functionalities. These structural constraints can be viewed as grammatical rules describing how to assemble parts together into larger functional units. Here, we develop a grammar for the design of synthetic transcription factors (sTFs) in eukaryotic cells and implement it within GenoCAD, a Computer-Aided Design (CAD) software for synthetic biology. Knowledge derived from experimental evidence was captured in this grammar to guide the user to create designer transcription factors that should operate as intended. The grammar can be easily updated and refined as our experience with using sTFs in different contexts increases. In combination with grammars that define other synthetic systems, we anticipate that this work will enable the more reliable, efficient, and automated design of synthetic cells with rich functionalities.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
Massachusetts Institute of Technology. Synthetic Biology Center
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/sb400134k
