Measuring chromosome conformation with degenerate labels
Name
Ross-2012-Measuring chromosome conformation with degenerate labels.pdf
Size
799.42 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Ross, Brian Christopher
Wiggins, Paul A.
Date Issued
July 2012
Journal
Physical Review E
Publisher
American Physical Society
Citation
Ross, Brian, and Paul Wiggins. “Measuring Chromosome Conformation with Degenerate Labels.” Physical Review E 86.1 (2012). ©2012 American Physical Society
Version
Final published version
Abstract
Although DNA conformation plays an integral role in all genetic processes from transcription to chromosome segregation, there is as yet no tractable method for capturing the in vivo conformation of a chromosome at high resolution. Labeling and fluorescently imaging thousands of loci along the chromosome would readily yield a conformation if each locus could be uniquely distinguished in the image, but this would unrealistically require thousands of distinguishable labels and a tedious experimental process. Here we present a computational method for extracting conformations when the total number of labels far exceeds the number of distinguishable labels. We evaluate our technique using simulated conformations with lengths ranging from 10 to 100 kilobases, and discuss the prospects for an experiment.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1103/PhysRevE.86.011918
