Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery
Name
77536995-MIT.pdf
Description
Full printable version
Size
9.05 MB
Format
Adobe PDF
Checksum (MD5)
82f549c7825183a213c7e62c13468d46
Author(s)
Was, Adam (Adam S.)
Advisor(s)
Richard T. Lee.
Alternative Title
Novel recombinant protein constructs for improved IGF-1 delivery
Date Issued
2006
Publisher
Massachusetts Institute of Technology
Abstract
Four novel recombinant protein constructs were designed to improve the delivery of insulin-like growth factor-1 (IGF-1). The IGF-1 sequence was subcloned into a HisMax plasmid and expressed at low levels by transient transfection in 293 cells. Site-specific mutagenesis was used to insert all four construct sequences into the IGF-1 HisMax plasmid, and to insert a secretory sequence into all four construct plasmids. A stable cell line selection in 293 and CHO cells using Zeocin was attempted. The four construct sequences were then subcloned from the HisMax plasmids to TrcHis plasmids and expressed in bacteria for higher production efficiency. Ni-NTA purified protein was detected in three of the four constructs. These proteins may ultimately be useful for myocardial delivery of IGF-1 to facilitate cardiac repair.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.
Includes bibliographical references (p. 87-89).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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