Dynamic pathway regulation: recent advances and methods of construction
Name
Tan et al_submitted.pdf
Description
Accepted version
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736.32 KB
Format
Adobe PDF
Checksum (MD5)
f4682e3ba3a5724505cf89dc7e5e944f
Author(s)
Tan, Sue Zanne
Prather, Kristala L
Date Issued
October 2017
Journal
Current Opinion in Chemical Biology
Publisher
Elsevier
Citation
Tan, Sue Zanne and Kristala L. J. Prather. "Dynamic pathway regulation: recent advances and methods of construction." Current Opinion in Chemical Biology 41 (December 2017): 28-35 © 2017 Elsevier Ltd
Version
Author's final manuscript
Abstract
Microbial cell factories are a renewable source for the production of biofuels and valuable chemicals. Dynamic pathway regulation has proved successful in improving production of molecules by balancing flux between growth of cells and production of metabolites. Systems for autonomous induction of pathway regulation are increasingly being developed, which include metabolite responsive promoters, biosensors, and quorum sensing systems. Since engineering such systems are dependent on the available methods for controlling protein abundance in the desired host, we review recent tools used for gene repression at the transcriptional, post-transcriptional and post-translational levels in Escherichia coli and Saccharomyces cerevisiae. These approaches may facilitate pathway engineering for biofuel and biochemical production.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.cbpa.2017.10.004
