7-Deazaguanine modifications protect phage DNA from host restriction systems
Name
s41467-019-13384-y.pdf
Description
Published version
Size
1.62 MB
Format
Adobe PDF
Checksum (MD5)
528568fc891ac50595f07437bb262d97
Author(s)
Hillebrand, Roman
Sassanfar, Mandana
Dedon, Peter C.
Date Issued
November 2019
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Hutinet, Geoffrey et al. “7-Deazaguanine modifications protect phage DNA from host restriction systems.” Nature Communications 10 (2019): 5442 © 2019 The Author(s)
Version
Final published version
Abstract
Genome modifications are central components of the continuous arms race between viruses and their hosts. The archaeosine base (G+), which was thought to be found only in archaeal tRNAs, was recently detected in genomic DNA of Enterobacteria phage 9g and was proposed to protect phage DNA from a wide variety of restriction enzymes. In this study, we identify three additional 2′-deoxy-7-deazaguanine modifications, which are all intermediates of the same pathway, in viruses: 2′-deoxy-7-amido-7-deazaguanine (dADG), 2′-deoxy-7-cyano-7-deazaguanine (dPreQ0) and 2′-deoxy-7- aminomethyl-7-deazaguanine (dPreQ1). We identify 180 phages or archaeal viruses that encode at least one of the enzymes of this pathway with an overrepresentation (60%) of viruses potentially infecting pathogenic microbial hosts. Genetic studies with the Escherichia phage CAjan show that DpdA is essential to insert the 7-deazaguanine base in phage genomic DNA and that 2′-deoxy-7-deazaguanine modifications protect phage DNA from host restriction enzymes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Center for Environmental Health Sciences
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41467-019-13384-y
