Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays
Name
Carter-Validation of Protei.pdf
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99.12 KB
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Author(s)
Carter, Bradley S.
Cortés-Campos, Christian
McCammon, Jasmine M.
Chen, Xiao
Sive, Hazel L.
Date Issued
August 2016
Journal
Zebrafish
Publisher
Mary Ann Liebert, Inc
Citation
Carter, Bradley S. et al. “Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays.” Zebrafish 14, 1 (February 2017): 73–76 © 2017 Mary Ann Liebert, Inc
Version
Final published version
Abstract
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1089/zeb.2016.1326
