Basal constriction during midbrain–hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase
Name
bio034520.full.pdf
Description
Published version
Size
15.88 MB
Format
Adobe PDF
Checksum (MD5)
d7a7d5eee569bceaef35a441c6fe2fb8
Author(s)
Gutzman, Jennifer H.
Graeden, Ellie
Brachmann, Isabel
Sive, Hazel
Date Issued
October 10, 2018
Journal
Biology open
Publisher
The Company of Biologists
Citation
Gutzman, Jennifer H., Ellie Graeden, Isabel Brachmann, Sayumi Yamazoe, James K. Chen, and Hazel Sive. "Basal constriction during midbrain–hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase." Biology open 7 (2018): bio034520 © 2018 The Author(s)
Version
Final published version
Abstract
Basal constriction occurs at the zebrafish midbrain-hindbrain boundary constriction (MHBC) and is likely a widespread morphogenetic mechanism. 3D reconstruction demonstrates that MHBC cells are wedge-shaped, and initially constrict basally, with subsequent apical expansion. wnt5b is expressed in the MHB and is required for basal constriction. Consistent with a requirement for this pathway, expression of dominant negative Gsk3β overcomes wnt5b knockdown. Immunostaining identifies focal adhesion kinase (Fak) as active in the MHB region, and knockdown demonstrates Fak is a regulator of basal constriction. Tissue specific knockdown further indicates that Fak functions cell autonomously within the MHBC. Fak acts downstream of wnt5b, suggesting that Wnt5b signals locally as an early step in basal constriction and acts together with more widespread Fak activation. This study delineates signaling pathways that regulate basal constriction during brain morphogenesis.
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1242/bio.034520
