Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules
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Author(s)
Syed, A.
Song, Y.-A.
Mangano, Lauren M.
Mao, Pan
Han, Joanna M.
Date Issued
December 2014
Journal
Lab Chip
Publisher
Royal Society of Chemistry, The
Citation
Syed, A. et al. “Creating Sub-50 Nm Nanofluidic Junctions in a PDMS Microchip via Self-Assembly Process of Colloidal Silica Beads for Electrokinetic Concentration of Biomolecules.” Lab Chip 14, 23 (2014): 4455–4460 © 2014 The Royal Society of Chemistry
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Author's final manuscript
Abstract
In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes.
MIT Department
Massachusetts Institute of Technology. Materials Processing Center
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1039/c4lc00895b
