Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells
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Author(s)
Guttman, Mitchell
Loewer, Sabine
Cabili, Moran N.
Loh, Yuin-Han
Thomas, Kelly
Park, In Hyun
Garber, Manuel
Curran, Matthew
Onder, Tamer T.
Agarwal, Suneet
Date Issued
November 2010
Journal
Nature Genetics
Publisher
Nature Publishing Group
Citation
Loewer, Sabine et al. “Large Intergenic Non-coding RNA-RoR Modulates Reprogramming of Human Induced Pluripotent Stem Cells.” Nature Genetics 42.12 (2010): 1113–1117.
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Author's final manuscript
Abstract
The conversion of lineage-committed cells to induced pluripotent stem cells (iPSCs) by reprogramming is accompanied by a global remodeling of the epigenome[superscript 1, 2, 3, 4, 5], resulting in altered patterns of gene expression[superscript 2, 6, 7, 8, 9]. Here we characterize the transcriptional reorganization of large intergenic non-coding RNAs (lincRNAs)[superscript 10, 11] that occurs upon derivation of human iPSCs and identify numerous lincRNAs whose expression is linked to pluripotency. Among these, we defined ten lincRNAs whose expression was elevated in iPSCs compared with embryonic stem cells, suggesting that their activation may promote the emergence of iPSCs. Supporting this, our results indicate that these lincRNAs are direct targets of key pluripotency transcription factors. Using loss-of-function and gain-of-function approaches, we found that one such lincRNA (lincRNA-RoR) modulates reprogramming, thus providing a first demonstration for critical functions of lincRNAs in the derivation of pluripotent stem cells.
Description
February 17, 2011
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/ng.710
