Label-free biomarker sensing in undiluted serum with suspended microchannel resonators
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Manalis-2010-Label-Free Biomarker Sensing in Undiluted Serum.pdf
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Author(s)
Brault, Norman D.
Jiang, Shaoyi
Von Muhlen, Marcio Goldani
Knudsen, Scott Michael
Manalis, Scott R.
Date Issued
February 2010
Journal
Analytical Chemistry
Publisher
American Chemical Society
Citation
von Muhlen, Marcio G. et al. “Label-Free Biomarker Sensing in Undiluted Serum with Suspended Microchannel Resonators.” Analytical Chemistry 82 (2010): 1905-1910. Web. 16 Nov. 2011. © 2010 American Chemical Society
Version
Author's final manuscript
Abstract
Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded microchannels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO[subscript 2] surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1021/ac9027356
